Abstract
To research the influence of tebufenozide and chlorbenzuron on the physiological mechanism of Apriona swainsoni (Hope), A. swainsoni larvae were fed with sawdust treated by chlorbenzuron (T1) and tebufenozide (T2). These were then sampled to determine enzymatic activity of catalase (CAT), superoxide dismutase (SOD), glutathione S transferases (GSTs), and carboxylesterase (CarE) in larvae at intervals of 12 h to 72 h in succession. Results showed that after treatment with chlorbenzuron, the enzymatic activity of CAT increased first and then decreased 24 h later. Enzymatic activity of SOD maintained a high level. Also, after treatment by tebufenozide, the enzymatic activity of CAT decreased first with enzymatic activity of SOD being higher than the control. After treatment by two types of the pesticides, enzymatic activity of GSTs was higher than the control with no differences of CarE activity with the control. Therefore, enzymatic activity of GSTs in A. swainsoni was assumed to be associated with resistance generated in the larva and was likely to be a resistance marker of the larvae.
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