Abstract
As one of the first steps toward developing a highly sensitive and specific radioimmunoassay for luteinizing hormone releasing factor (LRF), an antiserum to LRF was generated in female rabbits by repeated intradermal injections of LRF-bovine serum albumin (BSA) conjugate emulsified in Freund's complete adjuvant. After the third booster injection, 30% binding of 125I-LRF and a linear dose response relationship against cold LRF (0.25 ng to 10.0 ng) were obtained using a 1:1000 dilution of the antiserum. Compared with synthetic LRF, rat LH and FSH, substance P, oxytocin, lysine and arginine vasopressin, and synthetic TRF showed less than 0.03% displacement of LRF under these conditions. Intravenous injections of 1.0 ml of this antiserum to 4-day cycling female rats at 1:00 p.m. and 3:00 p.m. of the proestrous day blocked ovulation the next morning. The data indicate that this antiserum is appropriate for biological studies regarding gonadotropin release in addition to its use in developing a radioimmunoassay for LRF.
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