Oviposition behaviour and biochemical response of an insect pest, Zeugodacus cucurbitae (Coquillett) (Diptera: Tephritidae) to plant phenolic compound phloroglucinol

Abstract

Phenolic compounds are the secondary metabolites (SMs) present in plants carrying different bioactivities. In the present study, we explored the influence of a phenolic compound namely phloroglucinol on oviposition behaviour and different biochemical entities of an insect pest Zeugodacus cucurbitae (Coquillett) (Diptera: Tephritidae) using artificial diet. Phloroglucinol (IUPAC name: benzene-1,3,5-triol) affected the activity of antioxidant and detoxifying enzymes viz. superoxide dismutases (SOD), catalase (CAT), ascorbate peroxidases (APOX). dehydroascorbate reductase (DHAR), peroxidases (POX), phenol oxidase (PO), glutathione peroxidase (GPOX), glutathione S-transferase peroxidase (GSTpox), glutathione reductase (GR), glutathione S-transferase (GST) and esterases (EST) as well as the biological antioxidants viz. ascorbate content and glutathione. The lipid peroxide content (LP) and hydrogen peroxide content (H2O2) were significantly enhanced in the treated larvae indicating oxidative stress in the insect. Significant inhibition in oviposition was observed and effective repellency percentage increased with phloroglucinol treatment as compared to control. The oviposition deterrent activity and toxic effects of phloroglucinol on various biochemical parameters of Z. cucurbitae larvae revealed in the present study clearly confirms its suitability for use in pest management.