Ovicidal effectiveness of Hermecid-VS disinfectant against Trichuris spp. nematode eggs isolated from cattle

Abstract

The genus Trichuris is a well-known group of nematodes whose species parasitize humans and many domestic animals, including carnivores, cattle, small ruminants and pigs. These parasites in the embryonic stages of development are quite resistant to adverse environmental conditions, which poses a threat of significant spread of trichurosis in livestock farms. The aim of the work was to determine the ovicidal effectiveness of the Hermecid-VS disinfectant (Vetsintez LLC, Ukraine) on the eggs of Trichuris spp. nematodes isolated from cattle under experimental conditions. To determine the densivasive activity, the disinfectant was used in 0.1 %, 0.25 % and 0.5 % concentrations for different exposures – 10, 30 and 60 minutes. The test-culture of trichurises eggs was obtained from the feces of sick animals using the flotation method and subsequent washing in a physiological solution. According to the research results, it was established that the Hermecid-VS preparation has a high level of ovicidal efficiency against the eggs of Trichuris spp., which parasitize cattle, at a concentration of 0.25 % for exposure of 60 minutes and 0.5 % for exposures of 10–60 minutes . Under these regimes, the disinfectant ensured 100 % death of trichurises eggs at various stages of their development in vitro. Also, a high level of ovicidal efficiency was found when using Hermecid-VS at a concentration of 0.25 % for 10 min (90.91 %) and 30 min (96.59 %) exposures. A satisfactory level of ovicidal efficiency was obtained using the agent at a concentration of 0.1 % for exposures of 10, 30 and 60 minutes, where the indicators of disinfestation activity were at the level of 63.64 %, 68.18 % and 76.14 %, respectively. The obtained research results allow us to recommend the Hermecid-VS disinfectant in 0.25 % concentration for 60-minute exposure and 0.5 % concentration for 10-minute exposure for disinfestation as a component of measures to combat and prevent cattle trichurosis, as well as with for the purpose of maintaining veterinary-sanitary and epizootic well-being in livestock farms.