Abstract
Isolation of xylem-limited bacteria (XLB) on CS20 agar medium was used to assess bacterial survival in plum trees. Thirty to forty twigs were randomly sampled monthly from each of the three trees with plum leaf scald from October 1982 to October 1984. From December 1983 to May 1984, 16-24 roots were also sampled from three other trees and plated on CS20BN agar medium. Sap obtained by squeezing each surface-sterilized twig or root with pliers was immediately placed on the surface of CS20 or CS20BN agar. Plates were incubated at 30C and examined weekly with a microscope (50×) for bacterial colonies. Bacteria were isolated each month, except May 1983 and April 1984, from cultivar “Santa Rosa”; each month, except May 1983, February and April 1984, from cultivar “Burbank”; each month from July to December 1983 and August to October 1984 from cultivar “Ozark Premier”. XLB were isolated from root samples of each tree from November 1983 through May 1984. Results demonstrate that XLB can survive through winter at Byron, Georgia, and suggest that the XLB may be transmitted to healthy trees anytime vectors are present.
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