Abstract
Abstract The earlier theory of quasi-static linear gradient chromatography under overload conditions is improved. The quasi-crystalline phase model for the molecules adsorbed on the adsorbent surfaces in the column is specified; this model was introduced earlier, and it is used in the present theory. A close experimental verification of the theory is carried out for the case of hydroxyapatite high-performance liquid chromatography using both turkey and chicken egg white lysozymes as probes. It can be deduced that a quasi-crystalline phase of lysozyme molecules, in fact, is realized on the hydroxyapatite surfaces in the column. The mutual interaction energy per molecule of lysozyme occurring provided the maximum possible contact with other molecules is made on the hydroxyapatite surface can be estimated to be larger than 0.2–0.4 kcal/mol.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
