Abstract
Overexpression of the salicylic acid binding protein 2 (SABP2) gene from Tobacco results in enhanced tolerance to Huanglongbing (HLB; citrus greening disease) in transgenic sweet oranges. Huanglongbing (HLB), the most destructive citrus disease, is caused by Candidatus Liberibacter asiaticus (CaLas). Currently, no cure for this disease exists, and all commercially planted cultivars are highly susceptible. Salicylic Acid Binding Protein 2 (SABP2) is a well-characterized protein essential for establishing systemic acquired resistance (SAR) in tobacco.The constitutive over expression of SABP2 from tobacco (NtSABP2) in 'Hamlin' sweet orangeresulted in the production of several transgenic lines with variable transcript levels. Transient expression ofthe NtSABP2-EGFP fusion protein in Nicotiana benthamiana plants demonstrated thatNtSABP2was cytosolicin its subcellular localization. In a long-term fieldstudy, we identified a SABP2 transgenic linewithsignificantly reduced HLB symptoms that maintained aconsistently low CaLas titer. Transcriptome analysis of this selectedtransgenic line demonstrated upregulation of several genes related to plant defense and SAR pathways. Genes, such as NPR family genes and those coding for monooxygenases and lipoxygenases, were upregulated in the35S-NtSABP2 overexpressing line and might be candidates for incorporation into our citrus improvement program.
Published Version
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