Overexpression of Soybean Transcription Factors GmDof4 and GmDof11 Significantly Increase the Oleic Acid Content in Seed of Brassica napus L.

Qinfu Sun,Li Lin,Jinjin Jiang,Dongxiao Liu,Youping Wang,Jueyi Xue,Jian Wu

doi:10.3390/agronomy8100222

Abstract

Rapeseed (Brassica napus L.) with substantial lipid and oleic acid content is of great interest to rapeseed breeders. Overexpression of Glycine max transcription factors Dof4 and Dof11 increased lipid accumulation in Arabidopsis and microalgae, in addition to modifying the quantity of certain fatty acid components. Here, we report the involvement of GmDof4 and GmDof11 in regulating fatty acid composition in rapeseeds. Overexpression of GmDof4 and GmDof11 in rapeseed increased oleic acid content and reduced linoleic acid and linolenic acid. Both qPCR and the yeast one-hybrid assay indicated that GmDof4 activated the expression of FAB2 by directly binding to the cis-DNA element on its promoters, while GmDof11 directly inhibited the expression of FAD2. Thus, GmDof4 and GmDof11 might modify the oleic acid content in rapeseed by directly regulating the genes that are associated with fatty acid biosynthesis.

Highlights

Rapeseed (Brassica napus L.) is among the most important oil crops worldwide, providing high-quality edible oils and industrial raw materials [1,2,3]
Long-chain-CoA synthetase gene 5 (LACS5), respectively [44]. These results indicate that GmDof4 and GmDof11 can simultaneously increase seed oil content by upregulating genes that are involved in fatty acid synthesis and downregulating genes associated with the accumulation of seed protein in Arabidopsis
We found that GmDof4 and GmDof11 could activate or inhibit genes that are involved in fatty acid synthesis by directly binding to promoter regions

Summary

Introduction

Rapeseed (Brassica napus L.) is among the most important oil crops worldwide, providing high-quality edible oils and industrial raw materials [1,2,3]. Remarkable progress in increasing the content of seed oil and proportion of oleic acid has been reported by traditional breeding and putative candidate genes have been dissected using quantitative trait loci mapping and molecular markers [8,9,10,11]. Liu et al [20] overexpressed triacylglyceride (TAG) synthesis pathway genes in B. napus, including BnGPDH, BnGPAT, BnDGAT, and ScLPAAT, and found that the overexpression of a single gene could increase the content of seed oil, but the simultaneous overexpression of multiple genes may result in more substantial changes in oil composition

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Conclusion