Abstract
Low availability of phosphorus (P) in the soil is the principal limiting factor for the growth of cut chrysanthemum. Plant phosphate transporters (PTs) facilitate acquisition of inorganic phosphate (Pi) and its homeostasis within the plant. In the present study, CmPht1;2 of the Pht1 family was cloned from chrysanthemum. CmPht1;2 is composed of 12 transmembrane domains and localized to the plasma membrane. Expression of CmPht1;2 in roots was induced by Pi starvation. Chrysanthemum plants with overexpression of CmPht1;2 (Oe) showed higher Pi uptake, as compared to the wild type (WT), both under Pi-starvation and Pi-sufficient conditions, and also showed a higher root biomass compared to WT in the Pi-starvation conditions. Seven days after the P-deficiency treatment, 85 distinct analytes were identified in the roots and 27 in the shoots between the Oe1 plant and WT, in which sophorose, sorbitol (sugars), hydroxybutyric acid (organic acids), and ornithine (amino acid) of CmPht1;2 overexpressing chrysanthemum are specific responses to P-starvation.
Highlights
As one of the major macronutrients, phosphorus (P) plays an important role in plant biochemical synthesis, energy transport, and signal transduction pathways (Wang et al, 2017)
Various Pi transporters including the PHOSPHATE TRANSPORTER1 (Pht1) family are identified to function on the Pi uptake and translocation throughout the plants (Nagarajan et al, 2011)
We have identified the Pht1 family member CmPht1;2, whose expression is induced in the roots by P starvation
Summary
As one of the major macronutrients, phosphorus (P) plays an important role in plant biochemical synthesis, energy transport, and signal transduction pathways (Wang et al, 2017). Large amounts of phosphate-based fertilizer are applied in the agriculture system, most phosphorus in the soil becomes immobilized by precipitation or adsorption by soil minerals (Raghothama, 2000), resulting in a very low concentration of Pi in the soil solution available for plants (Vandamme et al, 2016). Nine Pi transporters in Arabidopsis thaliana were identified (Muchhal et al, 1996), and a Function of CmPht1;2 in Chrysanthemum number of homologs of PHT1 transporters have been isolated from other species (Rausch and Bucher, 2002). The functions of some Pht members have been identified.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
