Abstract
Rye and wheat can easily be crossed, providing the possibility to create disomic addition lines, which contain a pair of homologous chromosomes of rye in the genetic background of wheat. We obtained and used a set of Chinese Spring-Imperial (CS-IMP) disomic addition lines for facilitating the location of genes responsible for P starvation tolerance and root secretion of APase on the specific chromosome(s) of rye. A soil culture experiment showed that different chromosomes of Imperial rye in the background of common wheat Chinese Spring (CS) contributed differently to the trait investigated. Chromosome 1R and 7R of rye might carry genes responsible for tolerance to Pi starvation stress, while 5R might carry unfavorable genes regarding Pi starvation tolerance. APase activities of different disomic addition lines as well as background parent CS were 1∼12 and 2∼6 folds higher under Pi starvation conditions than under Pi sufficient conditions in terms of APase secretion on individual plant root system basis (µg/plant per h) and on unit dry root weight basis (µg/g per h) respectively. Among 7 disomic addition lines, DA1R secreted more APase than the rest of lines in both parameters mentioned above under Pi starvation condition. IEF (Iso-electric Focussing) diagram of APase isozymes clearly showed that chromosome 1R carries Pi starvation-inducible APase genes. Our results showed that genes conferring Pi starvation tolerance might be carried on 1R and 7R in the genome of rye, and the Pi starvation-inducible APase gene(s) carried in 1R might be one of the reasons of the better performance of line 1R under Pi starvation condition. Abbreviations: CS-IMP DA line – Chinese Spring-Imperial disomic addition line; APase – Acid phosphatase; MVGY – Mean Value of Individual Grain yield; RGY – Relative Grain Yield; IEF – Iso-electric Focussing; NPP – p-Nitrophenyl Phosphate; NP – p-Nitrophenol; Pi – inorganic orthophosphate
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