Abstract
We established a panel of 17 xenografts from primary human breast carcinomas. We examined which characteristics of the original tumours and the xenografts facilitate growth in animals. Tumours expressing medium or strong immunoreactivity for p53 protein had significantly (P < 0.05) higher incidence (92%) of in vivo tumour take than those showing weak or negative immunoreactivity (9.1%). No such association was observed between either c-erbB-2 or epidermal growth factor receptor (EGFR) expression in the original tumours and their in vivo tumour take. Following subcutaneous (s.c.) transplantation of original breast tumours or established xenografts, 7/17 tumours showed metastatic disease spread to distant sites (mainly lungs). This study suggests that selective growth of highly aggressive tumours occurs during in vivo propagation of malignant tumours, and these tumours will be of particular interest in evaluating various chemotherapeutic agents for breast cancer management.
Highlights
Sumaru We established a panel of 17 xenografts from primary human breast carcinomas
We examined which characteristics of the original tumours and the xenografts facilitate growth in animals
This study suggests that selective growth of highly aggressive tumours occurs during in vivo propagation of malignant tumours. and these tumours will be of particular interest in evaluating various chemotherapeutic agents for breast cancer management
Summary
We examined which characteristics of the original tumours and the xenografts facilitate growth in animals. Following subcutaneous (s.c.) transplantation of original breast tumours or established xenografts. Human tumour xenografts established in athymic mice provide important experimental material for cancer research. We achieved significant success in establishing human breast tumour xenograft lines in athymic mice Matrigel use increased tumour take, and enhanced tumour growth and facilitated distant metastasis (Mehta et al, 1993). Especially laminin and various growth factors, are directly involved in tumour angiogenesis It contains various growth factors generally present in basement membrane (Sabiston et al, 1985; Passaniti et al, 1992). Matrigel and laminin enhance the tumour take and promote growth of various human tumour types
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