Abstract
Autophagy is essential for plant growth, development, and stress resistance. However, the involvement of banana autophagy-related genes in drought stress response and the underlying mechanism remain elusive. In this study, we found that the transcripts of 10 banana ATG8s responded to drought stress in different ways, and MaATG8f with the highest transcript in response to drought stress among them was chosen for functional analysis. Overexpression of MaATG8f improved drought stress resistance in Arabidopsis, with lower malonaldehyde level and higher level of assimilation rate. On the one hand, overexpression of MaATG8f activated the activities of superoxide dismutase, catalase, and peroxidase under drought stress conditions, so as to regulate reactive oxygen species accumulation. On the other hand, MaATG8f-overexpressing lines exhibited higher endogenous abscisic acid (ABA) level and more sensitivity to abscisic acid. Notably, the autophagosomes as visualized by CaMV35S::GFP–MaATG8f was activated after ABA treatment. Taken together, overexpression of MaATG8f positively regulated plant drought stress resistance through modulating reactive oxygen species metabolism, abscisic acid biosynthesis, and autophagic activity.
Highlights
Drought stress is one of the most serious environmental threats, resulting in large yield loss in agriculture [1]
The transcript levels of MaATG8s were analyzed by quantitative real-time PCR (qRT-PCR) under drought stress of all,days the transcript levels of MaATG8s were analyzed under drought stressunder treatmentFirst for 0–25 (Figure 1)
The transcript level of MaATG8f showed under drought stress conditions by different ways
Summary
Drought stress is one of the most serious environmental threats, resulting in large yield loss in agriculture [1]. With the change of global warming, drought stress is becoming more and more serious through influencing the dynamic regulation between crops and soil [2]. In order to survive under adverse environmental stresses such as drought stress, plants have developed complex mechanisms [3]. Abscisic acid (ABA) is widely known for its involvement in plant drought stress responses. ABA was firstly found in wheat under drought stress conditions [4].
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