Abstract
NAC (no apical meristem (NAM), Arabidopsis thaliana transcription activation factor (ATAF1/2) and cup shaped cotyledon (CUC2)) transcription factors play crucial roles in plant development and stress responses. Nevertheless, to date, only a few reports regarding stress-related NAC genes are available in Malus baccata (L.) Borkh. In this study, the transcription factor MbNAC25 in M. baccata was isolated as a member of the plant-specific NAC family that regulates stress responses. Expression of MbNAC25 was induced by abiotic stresses such as drought, cold, high salinity and heat. The ORF of MbNAC25 is 1122 bp, encodes 373 amino acids and subcellular localization showed that MbNAC25 protein was localized in the nucleus. In addition, MbNAC25 was highly expressed in new leaves and stems using real-time PCR. To analyze the function of MbNAC25 in plants, we generated transgenic Arabidopsis plants that overexpressed MbNAC25. Under low-temperature stress (4 °C) and high-salt stress (200 mM NaCl), plants overexpressing MbNAC25 enhanced tolerance against cold and drought salinity conferring a higher survival rate than that of wild-type (WT). Correspondingly, the chlorophyll content, proline content, the activities of antioxidant enzymes superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) were significantly increased, while malondialdehyde (MDA) content was lower. These results indicated that the overexpression of MbNAC25 in Arabidopsis plants improved the tolerance to cold and salinity stress via enhanced scavenging capability of reactive oxygen species (ROS).
Highlights
Plants play a very important role in our life, but various stresses in the environment affect the normal growth and development of plants seriously
Previous reports have confirmed that NAC transcription factors (TFs) are involved in many plant growth and development processes such as apical meristem development, flower morphogenesis and lateral root development
NAC proteins are involved in plant defenses’ response to abiotic and Previous reports have confirmed that NAC TFs are involved in many plant growth and development processes such as apical meristem development, flower morphogenesis and lateral root development
Summary
Plants play a very important role in our life, but various stresses in the environment affect the normal growth and development of plants seriously. NAC transcription factors bind DNA or other protein kinases to regulate growth and development in plants and various physiological processes, including top meristem [11], secondary wall formation [12], flower organ development [13], bud differentiation [14], embryo development [15], lateral root formation [16,17], plant organ aging and fruit maturity [18]. Abiotic stress factors such as drought, high salt, cold and low temperature seriously affect the growth of plants. The function of the transcription factor was analyzed and identified to provide new candidate genes for cold and salt tolerance breeding
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