Overexpressed acetohydroxyacid reductoisomerase (ILV5) gene in Saccharomyces cerevisiae reduces diacetyl contents in Korean Campbell Early and Muscat Bailey a grape wines

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An ILV5 (acetohydroxyacid reductoisomerase) gene expression vector (designated pILV5) was constructed by cloning the yeast ILV5 gene into a yeast expression vector (pLG669Z) containing yeast CYC1 promoter. S. cerevisiae cells harboring the pILV5 plasmid showed ∼3.7-fold higher level of the diacetyl reductase activity compared with the control yeast cells. When they were applied to wine fermentation using Campbell Early and Muscat Baily A grapes, the diacetyl content level was reduced to ∼35.0–39.0% of those obtained with the control yeasts.