Over-expression and suppression of HMGB1 affect proliferation and invasion of endometrial carcinoma and underlying mechanisms

Abstract

To investigate effects of over-expression and suppression of HMGB1 on proliferation and invasion of endometrial carcinoma HEC-1A cell and underlying mechanisms. Methods: Over-expression or silence of HMGB1 in HEC-1A cell lines were established by lentiviral vector containing HMGB1 recombinant plasmid or by HMGB1 shRNA, respectively. Cell counting kit-8, Transwell, and wound healing assay were used to analyze proliferation, invasion, and migration of HEC-1A cells, respectively. Western blot and reverse transcription-PCR (RT-PCR) were used to detect the expression of NF-κB, VEGF, and matrix metalloproteinase 2 (MMP2) in the cells. Results: Over-expression of HMGB1 promoted the proliferation, invasion, and migration of HEC-1A cell, and up-regulated NF-κB, VEGF, and MMP2 expressions, while suppression of HMGB1 inhibited the proliferation, invasion, and migration of HEC-1A cells and down-regulated NF-κB, VEGF, and MMP2 expressions. Conclusion: HMGB1 plays an important role in proliferation, invasion, and migration of HEC-1A cell via NF-κB/VEGF/MMP2 pathway. HMGB1 might be a potential target for endometrial carcinoma therapy.