Abstract
Endometrial carcinoma is the most common gynecological malignancy in developed countries.Aim. With the aim to determine MMP-9 and TIMP-1 expression levels in the invasive endometrioid endometrial carcinoma (EEC) cells and areas of the immunopositive cells in the center of the tumor and in the area of tumor invasion into the myometrium pathohistological, immunohistochemical and morphometric study of histological specimens of operative material of 56 women with pT1-4 stage of tumor was performed.Methods and results. It was found that MMP-9 and TIMP-1 expression occurs in 100% of invasive EEC. The ММР-9 expression level in the cytoplasm of tumor cells is high – 129.11±2.38 CUOD, which is 2,2 times more than its level in normal proliferative endometrium – 59.45±9.58 CUOD (p <0.05 ). The TIMP-1 expression level is 9.58±2.68 CUOD that is 2.69 times more than the expression level of TIMP-1 in normal proliferative endometrium (3.56±0.35 CUOD, p <0.05) . MMP-9 immunopositive cells make up 50.78±0.91% of area of invasive EEC tissue section and TIMP-1 immunopositive cells make up 3.76±0.15% of area of tumor tissue section. That is statistically significantly more than in normal proliferative endometrium, which has 23.31±1.31% of ММР-9 immunopositive cells and 2.82±0.14% of TIMP-1 immunopositive cells of endometrial glands. Differences between high levels of MMP-9 in the center of EEC and in the tumor myometrial invasion zone, as well as differences between the very low expression levels of TIMP-1 in these regions of the tumor were not statistically significant. With increasing of EEC histological grade the MMP-9 and TIMP-1 expression levels significantly increase (p <0.05). There is direct weak correlation between the MMP-9 and TIMP-1 expression levels in the cells of EEC (Pearson coefficient r =+0.31). Direct strong correlation between the expression level of MMP-9 in the tumor cells and the grade of tumor (Pearson coefficient r =+0.71) is revealed.Conclusion. The results of the study showed the increasing invasive capacity of EAE with the tumor grade increasing.
Highlights
Аденокарцинома эндометрия является наиболее распространённым злокачественным новообразованием женских половых органов
It was found that matrix metalloproteinase - 9 (MMP-9) and TIMP-1 expression occurs in 100% of invasive endometrial carcinoma (EEC)
Of area of invasive EEC tissue section and TIMP-1 immunopositive cells make up 3.76±0.15% of area of tumor tissue section
Summary
С целью определения уровней экспрессии матриксной металлопротеиназы-9 (ММР-9) и её тканевого ингибитора TIMP-1 клетками инвазивной эндометриоидной аденокарциномы эндометрия (ЭАЭ), а также площади иммунопозитивных клеток в центре опухоли и в зоне её инвазии в миометрий патогистологическими, иммуногистохимическими и морфометрическими методами исследован операционный материал 56 женщин c pT1-4 стадией распространения опухоли. В центре ЭАЭ MMP9-иммунопозитивные клетки занимают 50,78±0,91% площади среза опухолевой ткани, а TIMP-1-иммунопозитивные клетки – 3,76±0,15%, что статистически достоверно больше площадей соответствующих иммунопозитивных клеток в ПЭ. Между уровнями экспрессии ММР-9 и TIMP-1 клетками ЭАЭ имеет место прямая слабая корреляционная связь. З метою визначення рівнів експресії матриксної металопротеїнази–9 (ММР-9) та її тканинного інгібітора TIMP-1 клітинами інвазивної ендометріоїдної аденокарциноми ендометрію (ЕАЕ), а також площі імунопозитивних клітин у центрі пухлини та в зоні її інвазії в міометрій патогістологічними, імуногістохімічними і морфометричними методами дослідили операційний матеріал 56 жінок із pT1-4 стадією поширення пухлини. Рівень експресії ММР-9 і TIMP-1 клітинами ЕАЕ статистично вірогідно зростає зі зменшенням гістологічного диференціювання пухлини від G1 до G3. With the aim to determine MMP-9 and TIMP-1 expression levels in the invasive endometrioid endometrial carcinoma (EEC) cells and areas of the immunopositive cells in the center of the tumor and in the area of tumor invasion into the myometrium pathohistological, immunohistochemical and morphometric study of histological specimens of operative material of 56 women with pT1-4 stage of tumor was performed
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
