Abstract
ObjectivesPolycystic ovary syndrome (PCOS) represents 75% of the cases of anovulatory infertility. The aim of this study was to investigate the role of aspirin on dehydroepiandrosterone (DHEA) - induced polycystic ovary syndrome in Wistar rats.MethodsTwenty eight (28) pre-pubertal female Wistar rats of 21 days old weighing 16 - 21 g were divided into 4 groups (7 rats/group) and treated as follows; group I received distilled water and served as Control; Group II received 6 mg/100 g body weight DHEA in 0.2 ml of oil subcutaneously to induce PCOS. Group III received 7.5 mg/kg of aspirin orally; Group IV received 6 mg/100kg of body weight of DHEA in 0.2ml of oil subcutaneously and 7.5 mg/kg of aspirin orally. After 15 days of administration, the rats were slaughtered by cervical dislocation. Blood samples and ovaries were collected for reproductive hormonal analysis, biochemical and histopathological analysis. The expressions of mRNA androgen receptor (AR) gene in the ovary were determined by real time reverse transcriptase polymerase chain reaction (qPCR). All the data was analyzed using one way ANOVA with the Graph pad prism software version 6. A p<0.05 was considered significant.ResultsThe results obtained showed that dehydroepiandrosterone treatment caused significant decrease (p<0.05) in total protein, superoxide Dismutase (SOD), glutathione-s- transferase (GST), Ca2+ ATPase, and significant increase (p<0.05) in malondialdehyde, vascular endothelial growth factor, tumor necrosis factor and estrogen as compared to Controls. The group co-administered with DHEA and aspirin showed significant increases in SOD, GST, CAT, GSH, Progesterone, Ca2+ ATPase, Na+ ATPase, H+ ATPase and significant reduction (p<0.05) in malondialdehyde, VEGF, TNF-α and estrogen as compared with the DHEA group. The histopathological analysis showed reductions in cystic fibrosis, atretic ovaries, increased expression of Bcl-2 and E- Cadherin and reduced Bax expression in the group that received Aspirin and DHEA.ConclusionThis study clearly demonstrates that Aspirin has ameliorating effects against polycystic ovary syndrome via anti-inflammatory and hormonal modulatory pathways.
Highlights
Infertility is a condition of the reproductive system, defined by the failure to achieve a clinical pregnancy after 12 months or more of regular unprotected sexual intercourse (Rowe et al, 1993)
Animal treatment and tissue collection Twenty-eight immature female Wistar rats (21 days old) were randomly divided into four groups (n=7): The rats in group I served as Control and were given distilled water daily, group II animals were injected with DHEA (6 mg/100 g in 0.2 ml corn oil subcutaneously daily) to induce Polycystic ovary syndrome (PCOS), group III was administered with Aspirin (7.5 mg/kg orally daily) and group IV was injected with DHEA (6 mg/100 g in 0.2 ml corn oil subcutaneously) and 7.5 mg/kg of Aspirin orally respectively
The results obtained showed that the group which received Aspirin and DHEA had a significant decrease (p
Summary
Infertility is a condition of the reproductive system, defined by the failure to achieve a clinical pregnancy after 12 months or more of regular unprotected sexual intercourse (Rowe et al, 1993). It may be caused by an underlying medical condition that may damage the fallopian tubes, interfere with ovulation, or cause hormonal complications. Ovarian dystrophy (physical damage to the ovaries, or ovaries with multiple cysts) and luteinized unruptured follicle syndrome (LUFS), in which case the egg may have matured properly but the follicle failed to burst, or it burst without releasing the egg may occur and cause an anovulatory cycle (Azziz et al, 2004). In PCOS the ovaries produce high amounts of androgens, testosterone, and amenorrhea or oligomenorrhea is quite common
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