Abstract
The purpose of this study is to investigate the outcomes of penetrating keratoplasty (PKP) following autologous cultivated limbal epithelial stem cell transplantation (CLET). A prospective, single center, interventional cohort study investigating patients with unilateral total limbal stem cell deficiency (LSCD) treated with CLET who underwent PKP. Patients with confirmed corneal re-epithelialization > 6 months post-CLET, and with best-corrected visual acuity (BCVA) <0.3 logMAR were offered PKP. CLET survival assessed by slit lamp, corneal impression cytology (CIC), and in vivo confocal microscopy. Confirmation of corneal re-epithelialization by histological and immunocytochemical (ICC) examination of trephined corneal buttons. Mean change in best-corrected visual acuity (logMAR) following PKP and PKP survival at 12 months were calculated. Twenty patients underwent PKP. Mean time of PKP was 19 months (range 11-41 months, SD 7.26) post-CLET. Median follow-up time post-PKP was 15 months (range 1-32, SD 10.2). CIC and ICC of all corneas confirmed corneal re-epithelialization before PKP. Mean pre-PKP BCVA was 1.46 (range 0.3-2.7, SD 0.94) improving to a mean post-PKP BCVA of 0.74 (range 0-2.7, SD 0.87); mean improvement in BCVA post-PKP of 36 letters (95% CI 15.0-57.1, p = .002). Kaplan-Meier mean graft survival was 90.9% (95% CI 50.8-98.7) at 12 months. We recommend a two-stage approach with CLET followed by PKP >12 months later. Patients experienced a significant improvement in BCVA following PKP. PKP did not have a detrimental effect on CLET survival. PKP survival post-CLET is better than that reported for high risk PKP. Stem Cells 2018;36:925-931.
Highlights
Limbal Stem Cell DeficiencyA clear cornea is essential for good vision
The purpose of this study is to investigate the outcomes of penetrating keratoplasty (PKP) following autologous cultivated limbal epithelial stem cell transplantation (CLET)
Twenty-three patients underwent CLET for unilateral total limbal stem cell deficiency (LSCD) as part of our Medical Research Council (MRC)-funded phase II clinical trial
Summary
Limbal Stem Cell DeficiencyA clear cornea is essential for good vision. In severe ocular surface diseases, such as chemical or thermal burns, there can be damage to limbal stem cells, resulting in limbal stem cell deficiency (LSCD). Limbal stem cells (LSCs) are located within crypts in a region known as the limbus, which forms the junction between the peripheral cornea and the sclera [1,2,3,4]. These cells are responsible for the continuous repair and renewal of the corneal epithelium. LSCD can be classified as mild/partial, in which only a limited part of the limbus is involved; or severe/total, in which more than two quadrants of the limbus are affected combined with central corneal involvement resulting in STEM CELLS 2018;36:925–931 www.StemCells.com
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