Abstract
BackgroundOrthodenticle homeobox 1 (OTX1) is a transcription factor that plays an important role in various human cancers. However, the function of OTX1 in laryngeal squamous cell carcinoma (LSCC) is largely unknown. We aimed to explore the roles of OTX1 in LSCC and its possible molecular mechanism.MethodsThe expression levels of OTX1 were assessed in LSCC cell lines and tissue samples. We further examined the effect of OTX1 on LSCC progression. The upstream regulator of OTX1 was identified using a computer algorithm and confirmed experimentally.ResultsOTX1 was highly expressed in 70.7% (70/99) of LSCC tissue samples. The OTX1 expression in LSCC was significantly correlated with lymph node metastasis. High OTX1 expression in patients with LSCC was correlated with poor prognosis. Knockdown of OTX1 inhibited proliferation, colony formation, migration and invasion in LSCC cells. Knockdown of OTX1 inhibited tumor growth in a xenograft mouse model. Mechanistically, OTX1 might act as a direct target of miR-129-5p. OTX1 enhanced tumorigenicity and tumor growth both in vitro and in vivo.ConclusionsOur findings support that OTX1 is an oncogene in LSCC tumorigenesis and progression. Furthermore, OTX1 is a direct target of miR-129-5p in LSCC cells. Taken together, OTX1 is a promising diagnostic and therapeutic marker for LSCC.
Highlights
Orthodenticle homeobox 1 (OTX1) is a transcription factor that plays an important role in various human cancers
OTX1 expression is correlated with the clinicopathological features and prognosis in patients with laryngeal squamous cell carcinoma (LSCC) To investigate the clinical significance of OTX1 expression in patients with LSCC, we first examined the expression levels of OTX1 in human LSCC tissues using IHC (Fig. 1)
The results revealed that high OTX1 expression was detected in 70.7% (70/99) of LSCC patient tissues
Summary
Orthodenticle homeobox 1 (OTX1) is a transcription factor that plays an important role in various human cancers. The function of OTX1 in laryngeal squamous cell carcinoma (LSCC) is largely unknown. We aimed to explore the roles of OTX1 in LSCC and its possible molecular mechanism. Laryngeal squamous cell carcinoma (LSCC) is one of the most common malignant cancers to occur in the head and neck region. With the continuous progression in studies, the role of OTX1 in the carcinogenesis and development of human cancer has drawn increasing attention. The activation of OTX1 expression occurs in aggressive non-Hodgkin lymphoma [20]. These findings suggest that OTX1 might be a potential oncogene and anti-cancer therapeutic target
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