Abstract
BackgroundThe aberrant expression of microRNAs (miRNAs) has been found in various types of cancer. miR-205 was reported to be upregulated in laryngeal squamous cell carcinoma (LSCC) tissues, however, the mechanisms by which miR-205 functions as a regulator of LSCC are largely unknown.ResultsIn this study, Real-time qPCR and Western blot assay showed that expression of miR-205 was upregulated and expression of cyclin-dependent kinase 2-associated protein 1 (CDK2AP1) was downregulated in LSCC tissues. The expression levels of miR-205 were negatively related to those of CDK2AP1 in LSCC tissues and cell lines. Moreover, we found that miR-205 was the upstream regulator of CDK2AP1 and could suppress the CDK2AP1 expression in LSCC cells. 3-(4,5-dimethylthiazal-2-yl)-2,5-diphenyl-tetrazolium bromide assays and transwell invasion assay were performed to test the proliferation and invasion of LSCC cells. Gelatin zymography was used to detect the activity of MMP2 and MMP9. CDK2AP1, c-Myc and CyclinD1 expression in cells was assessed with Western blotting. We found that miR-205 was the upstream regulator of CDK2AP1 and could suppress the expression of CDK2AP1 in LSCC cells. In addition, miR-205 significantly induced cell proliferation and invasion by suppressing CDK2AP1 expression. Consistent with miR-205 inhibitors, overexpressed CDK2AP1 suppressed the activity of MMP2 and MMP9 and c-Myc and CyclinD1 expression in LSCC cells.ConclusionThese findings help us to better elucidate the molecular mechanisms of LSCC progression and provide a new theoretical basis to further investigate miR-205 as a potential biomarker and a promising approach for LSCC treatment.
Highlights
The aberrant expression of microRNAs has been found in various types of cancer. miR-205 was reported to be upregulated in laryngeal squamous cell carcinoma (LSCC) tissues, the mechanisms by which miR-205 functions as a regulator of LSCC are largely unknown
Our finding indicates that the levels of miR-205 are negatively associated with those of cyclin-dependent kinase 2-associated protein 1 (CDK2AP1) mRNA in clinical LSCC tissues
MiR‐205 was the upstream regulator of CDK2AP1 Previous studies showed that miR-205 was upregulated in LSCC tissue [19]
Summary
The aberrant expression of microRNAs (miRNAs) has been found in various types of cancer. miR-205 was reported to be upregulated in laryngeal squamous cell carcinoma (LSCC) tissues, the mechanisms by which miR-205 functions as a regulator of LSCC are largely unknown. The aberrant expression of microRNAs (miRNAs) has been found in various types of cancer. MiR-205 was reported to be upregulated in laryngeal squamous cell carcinoma (LSCC) tissues, the mechanisms by which miR-205 functions as a regulator of LSCC are largely unknown. Laryngeal squamous cell carcinoma (LSCC) is one of the most common cancers with high incidence and mortality [1]. MicroRNAs (miRNAs), as a class of small (22-nucleotide) non-coding RNAs, have been identified to be aberrantly expressed in several human malignancies [4]. Previous studies have identified a number of miRNAs that show aberrant expression in LSCC [6,7,8,9]. MiR-129-5p is upregulated in primary LSCC tumors and functions as an oncogene in LSCC by repressing APC
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