Abstract
The properties and regulation of volume-activatedtaurine efflux from MDA-MB-231 and MCF-7 cellshave been investigated. Volume-activated taurinerelease from both cell lines was almost completelyinhibited by diidosalicylate. DIDS , was more effectiveat inhibiting swelling-induced taurine release fromMCF-7 than from MDA-MB-231 cells. On the basis ofcomparing taurine, Cl<sup>-</sup> and I<sup>-</sup> efflux time courses, itappears that volume-activated taurine efflux does notutilize volume-sensitive anion channels in MDA-MB-231 and MCF-7 cells. Extracellular ATP stimulatedvolume-activated taurine release from MDA-MB-231cells but not from MCF-7 cells. The effect of ATP wasmimicked by UTP and was dependent upon externalcalcium and inhibited by suramin. However, suramininhibited volume-activated taurine efflux from bothMDA-MB-231 and MCF-7 cells even in the absenceof exogenously added ATP suggesting that it actsdirectly on the taurine efflux pathway and/or is inhibitingthe effect of ATP released from the cells. Volumeactivatedtaurine efflux from MDA-MB-231 cells wasstimulated by ionomycin. In contrast, ionomycin hadno effect on taurine release from MCF-7 cells.Adenosine also stimulated volume-activated taurineefflux from MDA-MB-231 cells. The results suggestthat purines regulate taurine transport in MDA-MB-231 cells via more than one type of receptor.
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