Osmoregulation and Toxicity Test Ethanolic Extracts of Andaliman Leaves (Zanthoxylum acanthopodium D. C.) Against Physiological of Goldfish (Cyprinus carpio L.)

Abstract

The purpose of this study was to determine the osmoregulation of goldfish (Cyprinus carpio L.) treated with ethanolic extracts of andaliman leaves (Zanthoxylum acanthopodium DC) and to determine the toxicity level of ethanolic extracts of andaliman leaves (Zanthoxylum acanthopodium DC) on physiological goldfish (Cyprinus carpio L.). The method used in this study is included in experimental research with a significant correlation method to determine osmoregulation and toxicity testing ethanolic extracts of andaliman leaves (Zanthoxylum acanthopodium D. C.) on physiological goldfish (Cyprinus carpio L.). Measurements are made once at the same time. Quantitative chemical test results of the ethanol extract of andaliman leaves (Zanthoxylum acanthopodium D. C.) are phenol, hydroquinone, and amide compound. The results of this study indicate that there was a decrease in body weight of goldfish samples treated with ethanolic extracts of andaliman leaves compared with controls, and statistically showed significantly different. Then the results were also obtained that the higher the dose ethanolic extracts of andaliman leaves was given, the more the number of dead goldfish samples, where the 100% mortality dose was > 0.25 g/100 ml distilled water, and the length of time the goldfish died was also faster at time is 1-4 hours. For data on the number of erythrocyte goldfish samples that were treated with ethanolic extracts of andaliman leaves also decreased compared to controls, but statistically showed results that were not significantly different.