Origin and characterization of microglial cells in the avian central nervous system

Abstract

Event Abstract Back to Event Origin and characterization of microglial cells in the avian central nervous system Dávid Molnár1*, Imre Olah1 and Nándor Nagy1 1 Department of Human Morphology and Developmental Biology ,Semmelweis University, Hungary The origin of the microglial cells in the central nervous system and in the pineal gland is still a matter of debate. Besides the neuroepithelial origin recently the mesodermal or hemopoietic origin is generally accepted but not experimentally proved. The aim of our studies was to characterize immunocytochemically the microglial cells and to provide experimental evidence for their origin in the central nervous system. Different cell specific markers have been used for immunocytochemical characterization of the microglial cells: hemopoietic; B lymphocyte; T lymphocyte; macrophage; MHC class II, cell adhesion and avian dendritic cell. In the pineal parenchyma and over the CNS a poorly and highly ramified CD45+ hemopoietic cells can be distinguished. The former one expressed macrophage markers while other was positive for Bu1 (B cell specific antibody), CD44 and Ricinus communis agglutinin I (RCA I), a lectin specific for avian microglial cells. The immunocytochemical characterization of the CD45+/Bu1+/CD44+/RCA I+ cells strongly suggested their hemopoietic origin and expression of MHC class II antigen makes them capable for antigen presentation. To clarify the origin of microglial cells chick-quail chimera has been made: quail pineal gland from 10 days old embryo was isolated and transplanted either into the coelomic cavity of a 3 days old chicken embryo or onto the chorioallantioic membrane. Hemopoietic cells from the chick embryo migrated to and colonized the grafted pineal gland and differentiated into CD45+/Bu1+/MHC II+/CD44+/RCA1+ microglia cells. Conference: 12th Meeting of the Hungarian Neuroscience Society, Budapest, Hungary, 22 Jan - 24 Jan, 2009. Presentation Type: Poster Presentation Topic: Developmental neurobiology and subcortical functions Citation: Molnár D, Olah I and Nagy N (2009). Origin and characterization of microglial cells in the avian central nervous system. Front. Syst. Neurosci. Conference Abstract: 12th Meeting of the Hungarian Neuroscience Society. doi: 10.3389/conf.neuro.01.2009.04.067 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 02 Mar 2009; Published Online: 02 Mar 2009. * Correspondence: Dávid Molnár, Department of Human Morphology and Developmental Biology ,Semmelweis University, Budapest, Hungary, chewyd@freemail.hu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Dávid Molnár Imre Olah Nándor Nagy Google Dávid Molnár Imre Olah Nándor Nagy Google Scholar Dávid Molnár Imre Olah Nándor Nagy PubMed Dávid Molnár Imre Olah Nándor Nagy Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.