Abstract
The concentrations of the major and minor organophosphate metabolites of the rabbit and human crystalline lens were quantitated from perchloric acid extracts analyzed by phosphorus-31 nuclear magnetic resonance spectroscopy. The concentrations of adenosine diphosphate, phosphocreatine, inorganic orthophosphate and the nucleoside diphosphosugars were greater in the human lens samples when compared with the rabbit lens profile. A 10-fold greater phosphocretine content of the human lens was the most remarkable species difference. The adenosine triphosphate and the glycerol derivative, glycerol 3-phosphorylcholine, were enriched in the rabbit lens relative to the human. A prominent resonance signal at 6 p.p.m. corresonding to an as yet, unidentified phosphorus-containing molecule and two other less prominent phosphorus resonances of unidentified origin at 17·9 and 10·7 p.p.m., detected in the rabbit lens were absent from the human lens profile. In addition, the α-glycerophosphate content of the human lens was considerably less than that of the rabbit lens. These profile data document similarities and differences in the human versus the rabbit lens metabolites. The magnitude and diversity of these differences in lens metabolite concentrations emphasize the need for cautious rabbit to human extrapolation of experimental findings.
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