Abstract
Summary Embryo and organ differentiation in stem callus tissues derived from 5 members of the fam. Asclepiadaceae have been investigated. Pergularia minor formed embryoids and plant-lets, shoot buds and roots during the initial stages of callus growth but showed a decline in organogenetic potential with continuous subculture. In Asclepias curassavica , embryoids were differentiated at the senescent stage of the callus but these never grew into plantlets under any cultural variables. Dregea, Ibatia and Cryptostegia sp. formed profuse calli but lacked the ability to initiate organs.
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