Organization of cortical and subcortical projections to anterior cingulate cortex in the cat

Abstract

The aim of the experiments reported here was to identify cortical and subcortical forebrain structures from which anterior cingulate cortex (CGa) receives input in the cat. Deposits of retrograde tracers were placed at nine sites spanning the anterior cingulate area and patterns of retrograde transport were analyzed. Thalamic projections to CGa, in descending order of strength, originate in the anteromedial nucleus, lateroposterior nucleus, ventroanterior nucleus, rostral intralaminar complex, reuniens nucleus, mediodorsal nucleus, and laterodorsal nucleus. Minor and inconsistent ascending pathways arise in the paraventricular, parataenial, parafascicular, and subparafascicular thalamic nuclei. The basolateral nucleus of the amygdala, the hypothalamus, the nucleus of the diagonal band, and the claustrum are additional sources of ascending input. Cortical projections to CGa, in descending order of strength, derive from posterior cingulate cortex, prefrontal cortex, motor cortex (areas 4 and 6), parahippocampal cortex (entorhinal, perirhinal, postsubicular, parasubicular, and subicular areas), insular cortex, somesthetic cortex (areas 5 and SIV), and visual cortex (areas 7p, 20b, AMLS, PS and EPp). In general, the limbic, sensory, and motor afferents of CGa are weak. The dominant sources of input to CGa are other cortical areas with high-order functions. This finding calls into question the traditional characterization of cingulate cortex as a bridge between neocortical association areas and the limbic system.