Organization and phylogenetic relationships of the mitochondrial genomes of Speiredonia retorta and other lepidopteran insects

Yu Sun,Jian Geng,Yudong Liu,Hua Huang,Kai Zhang,Shanshan Liu,Jun Xia

doi:10.1038/s41598-021-82561-1

Yu Sun, Jian Geng + Show 5 more

Open Access

https://doi.org/10.1038/s41598-021-82561-1

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Abstract

In this study, we analyzed the complete mitochondrial genome (mitogenome) of Speiredonia retorta, which is a pest and a member of the Lepidoptera order. In total, the S. retorta mitogenome was found to contain 15,652 base pairs encoding 13 protein-coding genes (PCGs), 22 tRNAs, 2 rRNAs, as well as an adenine (A) + thymine (T)-rich region. These findings were consistent with the mitogenome composition of other lepidopterans, as we identified all 13 PCGs beginning at ATN codons. We also found that 11 PCGs terminated with canonical stop codons, whereas cox2 and nad4 exhibited incomplete termination codons. By analyzing the mitogenome of S. retorta using Bayesian inference (BI) and maximum likelihood (ML) models, we were able to further confirm that this species is a member of the Erebidae family.

Highlights

In this study, we analyzed the complete mitochondrial genome of Speiredonia retorta, which is a pest and a member of the Lepidoptera order
We found that the protein-coding genes (PCGs) of S. retorta contained all possible combinations of codons other than GCG, CGC, GGC, AGG, CCG, ACG, and TGG
We identified a full set of 22 tRNA genes (65–71 nucleotides long) in the S. retorta mitogenome, consistent with findings from other Lepidopterans

Summary

Introduction

We analyzed the complete mitochondrial genome (mitogenome) of Speiredonia retorta, which is a pest and a member of the Lepidoptera order. Given the clear value of such analyses, in the present study we sequenced the full S. retorta mitogenome in an effort to more fully explore the evolutionary relationship between this agriculturally important insect and other Noctuidae species.

Results

Conclusion