Abstract
This work reports new mixed matrix membranes (MMMs) for the adsorption of enzymes from organic solvents. In this work, polyimide/hydroxyapatite (PI/HAP) MMMs were prepared via phase inversion method and further crosslinked with 3-aminopropyl triethoxysilane (APTES). The chemical and structural stability of the crosslinked PI/HAP MMMs were improved and applied for lysozyme (LZ) adsorption in organic solvent. PI/HAP MMMs were crosslinked by changing the 3-aminopropyltriethoxysilane (APTES) concentration and crosslinking time. The optimal APTES crosslinking condition for PI/HAP MMMs is 6% of concentration for 8 h. The LZ adsorption performance was studied by changing solvent types. PI/HAP MMMs possessed a high LZ adsorption in organic-solvent-aqueous solutions, and the LZ adsorption capacity reached 34.1 mg/g. The MMMs had a high desorption capacity and recovery ability. The MMMs maintained 60% of their adsorption capacity and 58% of their desorption at the fourth cycle of adsorption and desorption. The MMMs provided a new technology for the purification and separation of enzymes or proteins by MMMs in organic solvents.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
