Organic chelates decrease phytotoxic effects and enhance chromium uptake by regulating chromium-speciation in castor bean (Ricinus communis L.).

Abstract

There is limited information available on changes in the uptake of essential nutrients and secondary metabolites accumulation in castor bean under Cr toxicity. Besides, the role of organic chelates (EDTA and citric acid) mediated improvement in Cr uptake by castor bean is mostly unknown. Three independent experiments (sand, hydroponics, and soil) were executed to determine the Cr phytoextraction potential of Ricinus communis L. In the sand experiment, optimum doses of organic chelates (EDTA and citric acid) were selected. These optimum doses of chelates were used in the hydroponics and soil experiments. The results of hydroponics and soil experiments manifested a significant decrease in growth characteristics and leaf pigments in response to Cr stress applied as K2Cr2O7 (a source of Cr6+). The application of organic chelates (2.5 and 5mM) showed a noticeable improvement in oxidative defense and secondary metabolites accumulation that might have decreased oxidative injury reflected as lower hydrogen peroxide (H2O2) and malondialdehyde (MDA) contents. Moreover, chelates improved the uptake of essential nutrients (K+, Ca2+, Mg2+, Fe2+ and P) alongside significant enhancement in total Cr contents of plants. Our results advocated that chelates application resulted in greater endogenous levels of Cr3+ in plants compared with Cr6+ which is more toxic. In nutshell, organic chelates improved growth by regulating Cr species, ion homeostasis and secondary metabolites accumulation in Ricinus communis L.