Abstract
in vivo. In our experiment mAb 263 showed 13.3% effect of GH maximum at concentration of 24.1 nM and its agonist effect could be significantly improved by the second antibodies. In contrast to mAb GHRA1, mAb 263 showed no antagonist effect. This study indicated that only GHR mAbs with epitopes on subdomain 1 are able to act as agonists in Baf/B03 cell proliferation assay. The fact that both mAb GHRA1 and mAb 263 could activate GHR implied that GH signaling could be induced through other ligands binding to a broader region of GHR than the GH binding site. However, a suitable conformation change of GHR similar as induced by GH might be needed.
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