Abstract
BackgroundChlamydomonas reinhardtii is an ideal model organism not only for the study of basic metabolic processes in both plants and animals but also the production of biofuels including hydrogen. Transgenic analysis of C. reinhardtii is now well established and very convenient, but inducible exogenous gene expression systems remain under-studied. The most commonly used heat shock-inducible system has serious effects on algal cell growth and is difficult and costly to control in large-scale culture. Previous studies of hydrogen photoproduction in Chlamydomonas also use this heat-inducible system to activate target gene transcription and hydrogen synthesis.ResultsHere we describe a blue light-inducible system with which we achieved optogenetic regulation of target gene expression in C. reinhardtii. This light-inducible system was engineered in a photosynthetic organism for the first time. The photo-inducible heterodimerizing proteins CRY2 and CIB1 were fused to VP16 transcription activation domain and the GAL4 DNA-binding domain, respectively. This scheme allows for transcription activation of the target gene downstream of the activation sequence in response to blue light. Using this system, we successfully engineered blue light-inducible hydrogen-producing transgenic alga. The transgenic alga was cultured under red light and grew approximately normally until logarithmic phase. When illuminated with blue light, the transgenic alga expressed the artificial miRNA targeting photosynthetic system D1 protein, and altered hydrogen production was observed.ConclusionsThe light-inducible system successfully activated the artificial miRNA and, consequently, regulation of its target gene under blue light. Moreover, hydrogen production was enhanced using this system, indicating a more convenient and efficient approach for gene expression regulation in large-scale microalgae cultivation. This optogenetic gene control system is a useful tool for gene regulation and also establishes a novel way to improve hydrogen production in green algae.
Highlights
Chlamydomonas reinhardtii is an ideal model organism for the study of basic metabolic processes in both plants and animals and the production of biofuels including hydrogen
Positive clones were purified and verified by sequencing. These results showed that the alga transformation was successful and that the transgenic algal strains contained both vectors required for the blue light-inducible exogenous gene expression system
Blue light‐inducible exogenous gene expression system The GAL4 DNA-binding domain (GAL4 BD) domain and VP16 transcription activator were fused with full-length CIB1 and CRY2, respectively
Summary
Chlamydomonas reinhardtii is an ideal model organism for the study of basic metabolic processes in both plants and animals and the production of biofuels including hydrogen. Since free algal cell separation and medium replacement are costly and inconvenient in large-scale culture, hydrogen photoproduction research has focused on extending the duration of hydrogen production in green algae without replacing the medium. The artificial miRNA successfully extended hydrogen production time, its expression was based on a heat-inducible system of Chlamydomonas reinhardtii, which affects algal cell growth. C. reinhardtii is a photosynthetic unicellular alga and has features inherited from the common ancestor of plants and animals that were subsequently lost in land plants It is an ideal model organism for studying many basic metabolic processes, such as chloroplast-based photosynthesis; the structure and function of eukaryotic flagella; and biofuels production [11,12,13]. We engineered a blue light-inducible expression system into alga in the present study
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