Abstract
Atemoya currently has its seeds studied in several aspects, from the technological and physiological point of view. However, for the performance of the germination test, there is no standardization in relation to the number of seeds and replicates. Thus, this work aims to determine the optimal sample size for germination tests with atemoya seeds. A germination test was carried out with 5 treatments, considering 10, 20, 30 40 and 50 seeds per sampling unit with 50 replicates of each. Data were analyzed using logistic regression, non-linear Gompertz regression models, bootstrap simulation and graphs in the form of contour lines in order to be able to infer the best binomial of the number of seeds per sampling unit and the number of replicates. The expected germination percentage, maximum germination speed and times for the beginning of the germination process, maximum germination speed and interruption of the germination process were determined. The treatment of 20 seeds per sampling unit statistically differs from that of 40 seeds by the Tukey-Kramer test with 5% significance. It could be concluded that for germination tests with atemoya seeds to have statistical validity, the smallest sample size is 10 seeds per unit, regardless of number of replicates.
Highlights
Annonaceae is a botanical family in which some species economically stand out due to their fruits that have high commercial value and generate interest in both domestic and external market
For germination tests, there is no standardization in relation to the number of seeds per sampling unit and number of replicates used: Carvalho et al (2018) used 5 replicates of 25 seeds in Annona × atemoya, Menegazzo et al (2012) performed tests with 4 replicates of 20 seeds of A. squamosa
The mean germination percentage reached with these treatments (20 and 40 seeds per s.u.) was not different from the others (10, 30 and 50 seeds) at 5% significance level by the Tukey-Kramer test
Summary
Annonaceae is a botanical family in which some species economically stand out due to their fruits that have high commercial value and generate interest in both domestic and external market. As well as the seeds of other Annonaceae, have been studied in several aspects, both from the technological point of view, for the production of seedlings and for the understanding of physiological processes, such as dormancy (Ferreira et al, 2016; Carvalho et al, 2018), desiccation tolerance (Corsato et al, 2013) and synthesis of specialized metabolites during germination (De La Cruz & González-Esquinca, 2012). For germination tests, there is no standardization in relation to the number of seeds per sampling unit and number of replicates used: Carvalho et al (2018) used 5 replicates of 25 seeds in Annona × atemoya (cv Thompson and Gefner), Menegazzo et al (2012) performed tests with 4 replicates of 20 seeds of A. squamosa. There are two factors to consider: number of seeds per sampling unit (pseudo-replicates) and number of sampling units to be used (replicates)
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