Abstract

The extraction of flavonoids from Osmanthus fragrans residues was optimized using ultrasonic device. The reaction parameters studied were the ethanol concentration (60-100%), the extraction time (1.5-2.5 h) and temperature (70-90°C) using response surface methodology based on Box-Behnken design. A mathematical model was developed to show the effects of each variable and their combinatorial interactions on extraction yield of flavonoids. A high coefficient of determination (R 2 = 99.57%) indicated good agreement between the experimental and predicted values of extraction yield. The optimal values of the variables were as the followings: ethanol concentration, 82%; extraction time, 2.1 h; and temperature 82°C. The ultrasound-assisted extractions supported higher extraction yields as compared to traditional extraction and could be recommended as an alternative method for extraction of flavonoids compounds from O. fragrans residue.

Highlights

  • Osmanthus fragrans Lour. is an evergreen tree, which is widely distributed in China, Japan and the Himalayas

  • The effects of several experimental parameters, such as ethanol concentration, extraction time and temperature, on the extraction efficiency of flavonoids from O. fragrans were optimized by Response surface methodology (RSM)

  • Single factor analysis method: To determine the single factor experiment condition, four main factors, including ethanol concentration, liquid-solid ratio, extraction time and temperature were studied in our test

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Summary

INTRODUCTION

Osmanthus fragrans Lour. is an evergreen tree, which is widely distributed in China, Japan and the Himalayas. O. fragrans is regard as one of the most famous traditional flowers of China and is widely cultivated as an ornamental plant today (Wang et al, 2006; Tsai et al, 2007). The plants which produce essential oil of the O. fragrans in China have increased year by year. The extraction process of essential oil is known to generate large quantities of O. fragrans residue, which retains high levels of flavonoids. It has been reported that RSM can be used to optimize the total flavonoid compound from many medicinal plants (Liu et al, 2010). The effects of several experimental parameters, such as ethanol concentration, extraction time and temperature, on the extraction efficiency of flavonoids from O. fragrans were optimized by RSM

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