Optimized DNA extraction method from skeletal remains using different typing methodologies in forensics

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In forensics DNA typing is usually based on STRs, but sometimes challenging samples demand the development of optimized DNA extraction methodologies. In addition, when DNA is severely degraded or in small amounts, results can be inconclusive. Thus another approaches, as miniSTRs, which consist on STRs redesigned to be more sensitive and generate smaller amplicons, are recommended. This study aimed to test an optimized, less time-expensive DNA extraction methodology for human bones combined with STRs and Mini-STRs on samples with previous inconclusive results. Our findings showed an efficiency improvement on both typing methodologies, which suggests that the optimized extraction method is promising.