Optimization ultrasound‐assisted extraction of anthocyanins from cranberry using response surface methodology coupled with genetic algorithm and identification anthocyanins with HPLC‐MS 2

Abstract

Cranberry contains abundant anthocyanins. In this study, the extraction process for the ultrasound-assisted extraction (UAE) of anthocyanins from cranberry was optimized using response surface methodology coupled with genetic algorithm. The optimum extraction parameters to achieve the highest yield of anthocyanins (7.25 ± 0.02) mg/g from cranberry via UAE were obtained at an extraction temperature of 53°C, ultrasound power of 310 W, ultrasound time of 33 min, and ethanol concentration of 52%. High-speed countercurrent chromatography was used to separate and enrich main anthocyanins from cranberry. The purified anthocyanin components were analyzed by HPLC-MS2. Seven major kinds of anthocyanins were detected and identified for the first time peonidin-3-(6-malon)-glucoside with a purity of 82.13% and pelargonidin-3-(6-malon)-glucoside with a purity of 85.37% in cranberry. Hence, ultrasound-assisted extraction of anthocyanins was an effective and rapid method. This method has a broad application prospect in the extraction of bioactive substances from natural plant resources. Practical applications Cranberries (Oxycoccos), as a good source of bioactive compounds, include anthocyanins, flavanols, various vitamins, superoxide dismutase, and phenolic acids, and the extraction process for the ultrasound-assisted extraction of anthocyanins from cranberry was optimized using response surface methodology coupled with genetic algorithm in this study. High-speed countercurrent chromatography was used to separate and enrich main anthocyanins from cranberry. This finding provides a new method for the extraction of anthocyanins from cranberry.