Optimization techniques: Studies in cell culture

Abstract

AbstractWith the present large worldwide demand for vaccines and cellular by products, a number of anchorage-dependent cell mass culture systems have been developed. To obtain the maximum yield of product per run, the number of cells per system volume must be maximized and yet maintain efficiency. There is a large number of independent variables that could affect the cell yield. During the course of an experimental program to increase the cell yield, the usefulness of an existing computer optimization program was evaluated.A series of nine statistically designed experiments was done using multi-disk propagators for the mass culture of primary chick embryo cells. Cell input and time between medium refeeds were chosen as the independent variables from the many variables that could affect cell yield. The primary dependent variable was cell yield. The results indicated a substantial increase in cell yield as cell input was increased (57 to 85 percent increase by doubling the cell input) but a lesser increase ...