Abstract
The Parker strain of Russian spring-summer encephalitis virus was maintained by serial passage in cell cultures of pig kidney, bovine embryo kidney and chick embryo. In pig kidney cell culture the virus proliferated and produced cytopathic changes. Bovine embryo kidney cell culture supported the proliferation of the virus but no cytopathic effect was produced. In chick embryo cell culture cytopathic changes, which were not shown in the early stages of passage, appeared after several passages. The virus adapted to this host system produced more acids in this culture under certain conditions. The virus interfered with Newcastle disease virus in cell cultures of swine kidney and bovine embryo kidney but not in chick embryo cell culture. The cytopathogenesis in chick embryo or pig kidney cell culture, the interference phenomenon with Newcastle disease virus in bovine embryo or pig kidney cell culture and the pH change in chick embryo cell culture could be employed as criteria for detecting and measuring the virus and its neutralizing antibody.
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