Abstract
Lagenaria siceraria (LS) is recognized for its ethnomedicinal properties. The present study aimed to compare and optimize extraction of bioactive compounds, total phenolic content (TPC) and total flavonoid content (TFC) from LS by microwave-assisted extraction (MAE), ultrasound-assisted extraction (UAE) and Soxhlet extraction (SE) using ethyl acetate as a green solvent. The fresh fruit was ground and dried under two methods freeze drying and oven drying. The dried biomass was defatted with hexane and the defatted material was used for extraction. Maximum % yield by UAE, MAE and SE was 5.08%, 4.00% and 3.20%, respectively. The TPC (mg/100 mg of GAE) had the overall order as 32.02 of UAE >31.99 of MAE >16.51 of SE. The overall trend of the TFC (mg/100 mg of RE) was 24.28 of MAE >21.94 of UAE >7.94 of SE. In conclusion, for polyphenolic compounds from L. siceraria, UAE and MAE were highly more powerful than SE.
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