Abstract
Celastrus hindsii is a potential source of flavonoids with biological activities. This study aimed to develop an ultrasound-assisted technique for extracting flavonoids from leaves of C. hindsii. Response surface methodology was employed to optimize the extraction conditions for maximizing the total flavonoid content (TFC). A maximum TFC of 23.6 mg QE/g was obtained under the extraction conditions of ultrasonic power of 130 W, extraction temperature of 40°C, extraction time of 29 min, and ethanol concentration of 65%. The flavonoid-rich extracts were then studied for their antioxidant and anticancer activities. The results showed that the C. hindsii leaf extract exhibited potent radical scavenging activities against DPPH (IC50 of 164.85 μg/mL) and ABTS (IC50 of 89.05 μg/mL). The extract also significantly inhibited the growth of 3 cancer cell lines MCF7, A549, and HeLa with the IC50 values of 88.1 μg/mL, 120.4 μg/mL, and 118.4 μg/mL, respectively. Notably, the extract had no cytotoxicity effect on HK2 normal kidney cell line. This study suggests that flavonoid-rich extract is a promising antioxidant and anticancer agent and that ultrasound-assisted extraction is an efficient method for extracting flavonoids from C. hindsii leaves.
Highlights
Cancer is a major health issue, which is ranked as a leading cause of mortality worldwide [1, 2]
Dulbecco’s Modified Eagle Medium (DMEM), Dulbecco’s Modified Eagle Medium/Nutrient Mixture F12 (DMEM/F12), fetal bovine serum (FBS), and antibiotics were obtained from Gibco (Waltham, MA, USA)
2.5. 2,2-Diphenyl-1-Picrylhydrazyl (DPPH) Assay. e free radical scavenging activity (RSA) of the C. hindsii leaf extract was determined using a DPPH test [28] with minor modifications
Summary
Dinh-Chuong Pham ,1 Hoang-Chinh Nguyen ,1 Thanh-Hang Le Nguyen, Hoang-Linh Ho, Thien-Kim Trinh, Jirawat Riyaphan, and Ching-Feng Weng. Celastrus hindsii is a potential source of flavonoids with biological activities. Is study aimed to develop an ultrasound-assisted technique for extracting flavonoids from leaves of C. hindsii. Response surface methodology was employed to optimize the extraction conditions for maximizing the total flavonoid content (TFC). E flavonoid-rich extracts were studied for their antioxidant and anticancer activities. E results showed that the C. hindsii leaf extract exhibited potent radical scavenging activities against DPPH (IC50 of 164.85 μg/mL) and ABTS (IC50 of 89.05 μg/mL). The extract had no cytotoxicity effect on HK2 normal kidney cell line. Is study suggests that flavonoid-rich extract is a promising antioxidant and anticancer agent and that ultrasound-assisted extraction is an efficient method for extracting flavonoids from C. hindsii leaves The extract had no cytotoxicity effect on HK2 normal kidney cell line. is study suggests that flavonoid-rich extract is a promising antioxidant and anticancer agent and that ultrasound-assisted extraction is an efficient method for extracting flavonoids from C. hindsii leaves
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