Optimization of Ultrasonic-Assisted Extraction of Chlorogenic Acid from Tobacco Waste.

Guoming Zeng,Xin Huang,Yonggang Ma,Yujie Ran,Libo Jin,Yan Li,Da Sun,Hongshuo Ma,Hui Ding,Maolan Zhang

doi:10.3390/ijerph19031555

Abstract

Using tobacco waste as raw material, the ultrasonic-assisted extraction of chlorogenic acid was optimized by response surface methodology (RSM). After repeated freezing and thawing of tobacco waste twice, the effect of pH value, ethanol volume fraction, temperature and extraction time on the extraction rate of chlorogenic acid was investigated by a single factor experiment. On the basis of this, the factors affecting the yield of chlorogenic acid were further optimized by using RSM. The optimum extraction conditions for chlorogenic acid were set at pH = 4.1, ethanol volume fraction was 49.57% and extraction time was 2.06 h. Under the above conditions, the extraction rate of chlorogenic acid could reach 0.502%, which was higher than traditional extraction and unpretreated ultrasonic extraction. All these results can be used as a reference for the extraction of effective ingredients in tobacco waste.

Highlights

IntroductionMost of it will be used to produce cigarettes, while about a quarter of the leftover material will be discarded and not reasonably utilized [1]
Most of it will be used to produce cigarettes, while about a quarter of the leftover material will be discarded and not reasonably utilized [1]. For this part of tobacco waste, if discarded casually, it may destroy the structure of the soil on the one hand; on the other hand, the harmful components in the tobacco waste will infiltrate into the groundwater, further harming human life [2]
Chlorogenic acid solutions of 0.5 mL, 1.0 mL, 1.5 mL, 2.0 mL, 2.5 mL and 3.0 mL were transferred into 25 mL brown volumetric bottles and the chlorogenic acid solutions of different concentrations were obtained by making volume with 50% ethanol

Summary

Introduction

Most of it will be used to produce cigarettes, while about a quarter of the leftover material will be discarded and not reasonably utilized [1]. For this part of tobacco waste, if discarded casually, it may destroy the structure of the soil on the one hand; on the other hand, the harmful components in the tobacco waste will infiltrate into the groundwater, further harming human life [2]. If these tobacco wastes can be reasonably used as resources, can the above problems be solved, and a large number of natural chemical raw materials can be obtained, and the income of tobacco farmers can be increased. It is in line with China’s policy on the resourcefulness of solid waste

Methods

Results

Conclusion