Optimization of Baicalin, Wogonoside, and Chlorogenic Acid Water Extraction Process from the Roots of Scutellariae Radix and Lonicerae japonicae Flos Using Response Surface Methodology (RSM)

Rui Wang,Zhiyong Wu,Jichang Li,Chunli Chen,Zunlai Sheng,Muhammad Ishfaq

doi:10.3390/pr7110854

Abstract

In this study, a simultaneous water extraction process for baicalin, wogonoside, and chlorogenic acid has been optimized. The effect of extraction temperature, extraction time, and liquid–solid ratio was scrutinized by single factor experiments and further analyzed by Box–Behnken design (BBD) approach using response surface methodology (RSM). The extraction yield of investigated compounds was determined by high performance liquid chromatography (HPLC). Single-factor experiments and response surface analysis results revealed that the optimized conditions are: Liquid to solid ratio 25:1 (mL/g), extraction temperature 93 °C, extraction time 2.4 h, and the extraction cycle two. Importantly, it has been noted that under the above conditions, concentrations of baicalin, wogonoside, and chlorogenic were 0.078, 0.031, and 0.013 mg/mL, respectively, and the overall desirability (OD) value was 0.76 which was higher than the non-optimized conditions and the deviation from the predicted OD value was only 2.44%. Conclusively, it has been suggested that the model was stable and feasible, and fit for extraction of baicalin, wogonoside, and chlorogenic acid from Scutellariae Radix and Lonicerae (L.) japonicae Flos.

Highlights

Scutellariae Radix is used in various pharmacological preparations in several East Asian countries [1].The active ingredients of this herbal plant contain several flavonoid compounds including wogonoside, baicalin, and their aglycone forms wogonin and baicalein [2]
In the current study, we focused on traditional water extraction process for the simultaneous extraction of baicalin, wogonoside, and chlorogenic acid
Of High chlorogenic acid, baicalin, and wogonoside in the sample appeared at retention

Summary

Introduction

Scutellariae Radix is used in various pharmacological preparations in several East Asian countries [1]. The active ingredients of this herbal plant contain several flavonoid compounds including wogonoside, baicalin, and their aglycone forms wogonin and baicalein [2]. Numerous studies have reported the anti-inflammatory, anti-oxidative, antiviral, and anti-tumor effects of baicalin [3]. The second major active ingredient in Scutellariae Radix is wogonoside, reported for its excellent anti-allergic, antioxidant, anti-inflammatory, and hepatoprotective activities [5]. The anti-inflammatory activity of wogonoside has been found due to the inhibition of TNF-α and IL-6 production in LPS-stimulated RAW264.7 cells [6]. Previous studies reported that Lonicerae (L.) japonicae Flos has Processes 2019, 7, 854; doi:10.3390/pr7110854 www.mdpi.com/journal/processes

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