The antioxidative and cytoprotective effect of Lonicerae japonicae Flos water extracts on the ultraviolet(UV)B-induced human HaCaT keratinocytes.

Abstract

ABSTRACT Objectives : Lonicerae japonicae Flos(LJF) has been reported to exhibit anti-oxidant, anti-infl ammatory, anti-viral, anti-rheumatoid properties. However, it is still largely unknown whether LJF inhibits the ultraviolet(UV)B-induced oxidative damage in human HaCaT keratinocytes. Therefore in thi s paper, we investigated the anti-oxidative capacity and protective effect of LJF against UVB-induced oxida tive demage in human HaCaT keratinocytes.Methods : To evaluate the anti-oxidative activity of LJF extracts, we measured total phenolic contents, total flavonoid contents, antioxidant capacity, and superoxide scavenging activity. To give an oxidative stress to HaCaT cells, UVB was irradiated with 200 mJ/㎠ to HaCaT cells. To detect the protective effect of LJF against UVB, we measured cell viability, DNA fragmentation and reactive oxygen species (ROS) production. In addition, we performed high-performance liquid chromatography (HPLC) analysi s to find a major component of LJF.Results : LJF contained phenolic and flavonoid contents, and showed the anti-oxidant and superoxide scavenging activity. The UVB-induced oxidative conditions led to the cell death, DNA fragmentation and reactive oxygen species (ROS) production. However, pretreatment with LJF reduce d oxidative conditions, including inhibition of cell death, DNA fragmentation and ROS production. In addition, we found out chlorogenic acid as major component of LJF.Conclusions : These results could suggest that LJF contained anti-oxidative contents and exhibited protective effects against UVB on human HaCaT keratinocytes. And the effective com pound of LJF which could show protective activities against UVB is chlorogenic acid. Thus, LJF and chlor ogenic acid would be useful for the development of drug or cosmetics treating skin troubles.Key words : HaCaT keratinocytes, Lonicerae japonicae Flos, UVB(ultravioletB), Reactive Oxygen Species (ROS), chlorogenic acid