Abstract
The culture conditions for the yield of a polysaccharide (PCPS) produced by Paecilomyces cicadae (Miquel) Samson on solid-state fermentation were investigated using response surface methodology (RSM). Plackett–Burman design (PBD) was applied to screen out significant factors, followed by the paths of steepest ascent to move to the nearest region of maximum response. Then Box-Behnken design (BBD) was conducted to optimize the final levels of the culture conditions. After analyzing the regression equation and the response surface contour plots, relative humidity 56.07%, inoculum 13.51 mL/100 g and temperature 27.09°C were found to be the optimal key parameters for PCPS production. The maximum predicted yield of PCPS was 10.76 mg/g under the optimized conditions. The resulting PCPS (FPCPS) generated at optimal conditions was purified by chromatography column and found to be composed of mannose (43.2%), rhamnose (32.1%), xylose (14.5%) and arabinose (10.2%). Based on the size exclusion chromatography combined with multi-angle laser light scattering (SEC-MALLS) analysis, FPCPS adopted a Gaussian coil conformation in 0.1 M NaNO3 solution with 3.75×106 g/mol of the weight-average molar mass (Mw) and 41.1 nm of the root-mean square radius (Rg2)z 1/2. Furthermore, both of the polysaccharides were revealed to have strong antioxidant activities by evaluating in DPPH radical, superoxide radicals and hydroxyl radical assay. These data suggest the polysaccharides of Paecilomyces cicadae (Miquel) Samson produced by solid-state fermentation could be explored as potential natural antioxidants.
Highlights
Medicinal fungi can secrete sorts of important secondary metabolic products, which have a wide range of applications in pharmaceutical and food industries
Based on the size exclusion chromatography combined with multi-angle laser light scattering (SEC-MALLS) analysis, FPCPS adopted a Gaussian coil conformation in 0.1 M NaNO3 solution with 3.756106 g/mol of the weight-average molar mass (Mw) and 41.1 nm of the root-mean square radius (Rg2)z1/2
Both of the polysaccharides were revealed to have strong antioxidant activities by evaluating in DPPH radical, superoxide radicals and hydroxyl radical assay. These data suggest the polysaccharides of Paecilomyces cicadae (Miquel) Samson produced by solidstate fermentation could be explored as potential natural antioxidants
Summary
Medicinal fungi can secrete sorts of important secondary metabolic products, which have a wide range of applications in pharmaceutical and food industries. Polysaccharides have been well studied due to their novel functionality, constant reproduction and stable cost [1]. They can be incorporated in food industry as thickeners and bio-emulsifiers to improve food quality and texture [2]. Polysaccharides with different structures have been found to exist as various chain conformations in solution [6]. The main aim of this work was to optimize the solidstate fermentation conditions of P. cicadae based on the statistical analysis and to elucidate the characterization of highly purified soluble polysaccharide and evaluate its antioxidant activities, which will provide the foundation for future pharmacological and biochemical studies
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