Abstract
The purpose of the work was to rise of biosynthesis of the human recombinant interleukin-7 (rIL-7) in E. coli by optimizing the quantitative and qualitative composition of the culture medium. As a result of optimization of the quantitative composition of organic and mineral components of the culture medium using the matrix of planning a fractional factor experiment, it was possible to increase the synthesis of rIL-7 by 1.3 times. It was proved that the use of supplements to the culture medium in the form of extracts of Clivia miniata and Zephyranthes grandiflora (0.5-1.0%) stimulated the rIL-7 synthesis in recombinant bacterial cells in 1.3-1.4 times. The use of phytoextracts at a concentration of 5.0% and more caused an inhibitory effect on the producer biosynthetic properties. It has been established that the modulation of the biosynthetic activity of recombinant bacteria is due to the interaction of the biologically active substances of plant extracts with the surface structures of the bacterial cell, namely, porins (OmpC and OmpF proteins). The stimulating effect of vitamin K1 (15-25 mg per 1 ml of culture medium) in the liposomal form was proved; such approach allowed increasing the synthesis of rIL-7 in 1.34-1.39 times. And the use of liposomal form of plant extracts as an approach to increasing the bioavailability of biologically active substances is not appropriate.
Highlights
Optimization of biosynthesis processes of recombinant medical products is an actual problem of biotechnology
The analysis of the above literary sources led us to the idea of the expediency of evaluating the application of extracts of such plants in the culture medium when cultivating E. coli to increase the yield of recombinant interleukin-7 (rIL-7) of the following supplements: the extracts of Clivia miniata and Zephyranthes grandiflora, as well as vitamin K1
The determined rational ratio of organic and mineral components allowed increasing the yield of biomass by 1.4 times and the synthesis of rIL-7 by 1.3 times compared to the base composition
Summary
Optimization of biosynthesis processes of recombinant medical products is an actual problem of biotechnology. The works of various authors testify to the possibility of effective application of plant extracts as growth stimulators of bacterial cells, as well as the synthesis of bacteria of certain products, including recombinant proteins (Pererva et al, 2012; Galkіn et al, 2015; Pererva et al, 2012). The aim of the work was to optimize the composition of the culture medium through the use of minerals and organic compounds, studying the influence of Clivia miniata and Zephyranthes grandiflora extracts on the level of biosynthesis of recombinant interleukin-7 by E. coli bacteria, as well as studying possible mechanisms of interaction of plant substances with a bacterial cell
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of microbiology, biotechnology and food sciences
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
