Abstract
Aims: The aims of this work was optimization of two -step enzymatic hydrolysis of sweet potato starch using statistical approach and subsequent utilization of the hydrolyzate obtained for citric acid production. Methodology: Box Behnken design was used in this s tudy to generate a total of 17 individual experiments for each step of the hydrolysis (liquefaction and saccharification steps). These were designed to study the effect of temperature, time and pH on the sweet potato starch hydrolyzate (SPSH) concentration . The optimization was carried out using response surface methodology (RSM). The SPSH obtained was used to culture Aspergillus nigerfor citric acid production. Results:A statistically significant quadratic regression model (P<
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