Optimization of simultaneous saccharification and fermentation in bioethanol production from sugarcane bagasse hydrolyse by Saccharomyces cerevisiae BTCC 3 using response surface methodology

Abstract

The response surface method (RSM) was used to investigate the effects of bioethanol fermentation parameters, including sugarcane bagasse concentrations (0-5 % (w/v), enzyme types (cellulase and hemicellulase), enzyme concentrations (0-1 FPU/g biomass), medium pH, and incubation period. Bioethanol production was conducted by simultaneous saccharification and fermentation (SSF) of sugarcane bagasse hydrolyse using Saccharomyces cerevisiae BTCC 3. The experiment results showed that the maximum bioethanol production by using the SSF method was the same as RSM prediction. The optimum conditions for bioethanol production were 5% (w/v) of sugarcane bagasse, a mixture of cellulase and hemicellulase at 1 FPU/g of sugarcane bagasse for each enzyme, pH medium 6.0, and fermentation period 72 hours. The bioethanol yield of 2.43 g/L was obtained under these conditions.