Optimization of bioethanol production from simultaneous saccharification and fermentation of pineapple peels using Saccharomyces cerevisiae

O Oiwoh,B.V Ayodele,N.A Amenaghawon,C.O Okieimen

doi:10.4314/jasem.v22i1.10

Abstract

In this study, bioethanol production from the simultaneous saccharification and fermentation (SSF) of pineapple peels using cellulase and Saccharomyces cerevisiae was investigated. A three-factor Box-behnken design (BBD) and response surface methodology (RSM) were employed to study the effect of broth pH (2-6), yeast loading (2- 10 g/l) and ammonium sulphate concentration (1-5 g/l) on the bioethanol production process. Optimum values of pH, yeast loading and ammonium sulphate concentration of 6.0, 8ml and 5g/l, respectively were obtained for maximum bioethanol concentration of 5.82%v/v. The results obtained show the possibility of using pineapple peels as feedstock for bioethanol production via SSF method. Moreover, the use of BBD and RSM as robust technique for determining the effect of parameters and optimum conditions for bioethanol production has been ascertained.Keywords: Bioethanol; Box-behnken design; Fermentation; Optimization; Pineapple peels

Highlights

The excessive consumption of non-renewable energy has greatly resulted in environmental deterioration and public health problems (Kahia et al, 2016)
Response surface methodology (RSM) which is a useful tool that helps to identify the effects of several process variables influencing a particular response by varying them simultaneously and carrying out a limited number of experimental runs is more commonly employed. (Cazetta et al, 2007) The objective of this study was to optimize important parameters for the bioethanol production from pineapple peels via simultaneous hydrolysis and fermentation using Saccharomyces cerevisiae
The use of Box-behnken design (BBD) in this study resulted in a non-linear second order model between the input variables (pH, yeast loading and (NH4)2SO4 concentration) and the output variable

Summary

Introduction

The excessive consumption of non-renewable energy has greatly resulted in environmental deterioration and public health problems (Kahia et al, 2016). There are a lot of unused excess parts of the pineapple, notably the peels, which are considered as waste and contribute to the country’s garbage problem. These peels are a major component of domestic and industrial waste worldwide, rich in sugar and lignocellulosic components and account for 29-40% (w/w) of the total pineapple weight. (Cazetta et al, 2007) The objective of this study was to optimize important parameters for the bioethanol production from pineapple peels via simultaneous hydrolysis and fermentation using Saccharomyces cerevisiae Response surface methodology (RSM) which is a useful tool that helps to identify the effects of several process variables influencing a particular response by varying them simultaneously and carrying out a limited number of experimental runs is more commonly employed. (Cazetta et al, 2007) The objective of this study was to optimize important parameters for the bioethanol production from pineapple peels via simultaneous hydrolysis and fermentation using Saccharomyces cerevisiae

Objectives

Methods

Results

Conclusion