Optimization of Simultaneous Analysis of Cefixime and Dicloxacillin Sodium in Oral Tablets

Abstract

The present manuscript describes simple, sensitive, rapid, accurate, precise and economical spectrophotometric method for the simultaneous determination of cefixime and dicloxacillin sodium in their combined tablet dosage form. The method was based on additive property of absorbance and correction of absorbance for the analysis of two drugs using methanol as solvent. The two wavelengths were selected from the UV spectra of both the drugs, which 218.4 nm and 289 nm. Cefixime was determined directly at 289 nm (λmax of cefixime) without any interference of dicloxacillin sodium in binary mixture. At 218.4 nm, both drugs have reasonable absorbance. So to remove interference of cefixime, its absorbance was calculated by using its standard absorptivity values at this wavelength. Finally, corrected absorbance of dicloxacillin sodium at 218.4 nm was found by subtracting the absorbance of cefixime from the total absorbance at 218.4 nm. The linearity was found in the concentration range of 3-16 μg/ml for both the drugs. The % recovery was found in the range of 100.22 ± 0.43 and 100.46 ± 0.32 for cefixime and dicloxacillin sodium respectively. The intermediate precision data obtained under different experimental setup, the calculated value of % coefficient of variation (% CV) was found to be less than critical value. The method was successfully applied for the simultaneous determination of these two drugs from their combined tablet dosage form without any interference.