Optimization of Protoplast Isolation and Micropropagation Techniques in Rose

Abstract

Rose is the dominant flower in the ornamental industry because of its multiple aesthetic values. Optimization of plant growth regulators in media was achieved for one of the important species of rose. The nodal explants were tested on a full strength MS media with different hormone concentrations of 6-benzylaminopurine (BAP) and Kinetin (Kn). Maximum response was obtained for a combined hormone concentration of 6-benzylaminopurine (3.5mg/L) and kinetin (1.5mg/L) with shoot regeneration from nodes, internodes and apical meristematic tissue. 6-benzylaminopurine alone showed shoot regeneration only from internodes and nodes, while kinetin alone showed shoot regeneration only from apical meristematic tissue. Protoplast of Rosa sp. and Rosa sinensis were isolated enzymatically and fused using polyethylene. The protoplasts were observed to fuse on different planes in an irregular pattern.