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Abstract
Background: Leaf protein concentrate (LPC) is a cheap and feasible source of high-quality protein. Researchers found the protein quality of LPC is similar to first-class protein food. Tobacco is a protein-rich leaf but contains a high amount of nicotine. According to studies the leaf protein concentrate obtained from tobacco leaves is nicotine-free and also useful to gaining weight in malnourished rats. Methods: Fresh leaves were blended with distilled water in different leaf water ratio (1:3, 1:6,1:9 and 1;12). The juice was filtered and coagulated at different pH (acidic, basic and neutral) with different coagulating techniques (conventional heating, Microwave and Ultrasound) to standardize the optimum procedure for leaf protein concentrate extraction. The coagulant was filtered and dried in the oven. Result: The maximum quantity of crude protein and LPC yield (62.34% and 4.7 g) was extracted with a 1:9 leaf water ratio at acidic pH by using citric acid followed by the conventional heating method.
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